内容如下:请不要软件翻译。量有点多。给最高分。
Operation
Complete, easy-to-follow instructions, including buffer
preparation, sample preparation and purification protocol
are included with MAbTrap Kit.
Just a few short steps will give you purified IgG. A summary
of the method follows.
1. Allow column and buffers to warm to room temperature.
2. Dilute buffers and prepare sample.
3. Connect the syringe to the
column through the Luer
adapter.
4. Equilibrate the column with
5 ml distilled H2O and 3 ml
diluted binding buffer
(Fig 2a).
5. Apply the sample (Fig 2b).
6. Wash with 5-10 ml diluted
binding buffer until no
material appears in the
effluent.
7. Elute with 3-5 ml diluted
elution buffer. Collect
fractions into tubes
containing neutralizing
buffer (Fig 2c).
8. Re-equilibrate the column
with 5 ml diluted binding
buffer.
9. Store the column in 20%
ethanol at 4°C to 8°C.
10. Store MAbTrap Kit at 4°C to 8°C
是长了点。但物有所值。只需要翻译1-10条就可
对不起,我不需要,网上机器翻译。这个翻译我也会。
11楼和3楼的兄弟,我希望懂得人帮我。不懂就不要在这想得2分